500 – Internal Server Error Message

We’ve created a new AWLRS support web site as a clone of our AWLRS production web site. Everything seems ok, but the map layers aren’t being displayed when logging into the support web site. Below is a screenshot of the error messages we are getting:

Here is an entry from the AWLRS site log:

The map files are getting created on the map server:

Any help to resolve this problem would be greatly appreciated.